Semen is collected on the day of the ovum pick up and the male partner must contact the reproductive biologist to determine what time they are required to give the sample .The semen is generally processed using the double wah and swim up method.Depending on the semen report semen can be used for IVF or ICSI.
ICSI – Intra Cytoplasmic Sperm Injection :
The direct injection of sperms into the eggs obtained after ovum pick up frequently permits the establishment if pregnancy in even the most difficult types of  male infertility including men who have no sperms in the semen sample because of vasectomy, blocked ducts failure to ejaculate because of spinal cord injury , non obstructive azospermia and conjential absence of vas a non surgical sperm aspiration is performed .TESA /PESA is an easy and quick method to obtain adequate number of sperms for ICSI. TESA .PESA is performed under sedation and is painless and rapid .a tiny needle is used to extract sperms directly from the testis. Aspiration of 100/ 200 sperms by is enough to achieve pregnancy when combined with ICSI .

IVF:
In a natural cycle fertilization occurs in the outer part of the fallopian tube. In a IVF cycle the harvested eggs are mixed with sperms ( 1 lac sperms/egg ) in a Petri dish. Fertilization is usually checked after 16-18hrs. IVF is generally done for patients in whom the semwn parameters are normal.

CAUSES OF CANCELLATION OF TREATMENT CYCLE PRIOR TO OPU:

The aim intensive monitoring programme is to obtain healthy mature eggs ,Thus if the cycle is unsatisfactory. It may be cancelled at any stage.

1) The follicles are developing in a necessary ovary .
2) When very few follicles are developing which would decrease the chance of obtaining at least one mature egg.
3)There are no follicles developing at all. This means higher doses must be administrated next time.
4) The blood oestrogen levels are abnormal ,they  may be too low or too high for the development of healthy eggs or according to the patterns seen over the last 5 years unlikely to lead to pregnancy
5) The follicles may have ovulated prior to ovum pick up .Occasionally some women ovulate earlier than expected or ovulate without our being able to detect the time when ovulation commenced.
6) Sometimes ovarian cysts develop in response to the drugs .These are neither serious nor harmful but the cycle may have to be abandoned as it is difficult to satisfactory monitor the growth of other follicles or the cyst may have to be punctured .they usually resolve within one month .
7) Rarely . due to circumstances beyond our control, such as equipment overload , equipment failure or patient overload , you may be advised that your current treatment cycle is to be abandoned.